FIG. 7.

VP22 partitioning in the nuclear fractions of HSV-1(F)-infected cells does not require viral DNA or true late protein synthesis. Vero cells were synchronously mock (M) or HSV-1(F) infected in the absence (−) or presence (+) of PAA. At 13 hpi, whole-cell, nuclear, and cytoplasmic fractions (Extract) were prepared, and polypeptides were separated in denaturing gels, transferred to nitrocellulose, and probed with antibodies to VP22, VP13/14, and ICP4 as described in Materials and Methods. Locations of the migrations of molecular mass markers are indicated in the right margin.