FIG. 8.

Indirect immunofluorescence of VP22 during HSV-1(R7032) infection in the presence of PAA. Vero cells were synchronously mock or HSV-1(R7032) infected with (+) or without (−) PAA, fixed for indirect immunofluorescence at the times indicated, and stained with antibodies to α-tubulin and VP22 as described in Materials and Methods. White arrowheads mark cells referred to in the text. Merged images (overlay) are shown in panels D, H, L, P, T, and X. All images were acquired under the same conditions.