. 2024 Aug 17;416(27):6149–6159. doi: 10.1007/s00216-024-05483-7

Table 1.

Urinary glucose estimation in volunteer urine samples using the gold standard enzyme-only assay and the Ag-Pt nanozyme fabric assay

Volunteer urine samples	GOx-HRP approach^a	Ag-Pt fabric approach^b
Volunteer urine samples	Estimated urine glucose conc./mM^c,e	Estimated urine glucose conc./mM^d,e	Recovery/%^f
Healthy—1	0.0 ± 0.00^g	0.0 ± 0.00	100
Healthy—2	0.0 ± 0.00^g	0.0 ± 0.00	100
Diabetic—1	3.13 ± 0.1	3.2 ± 0.09	102–103
Diabetic—2	1.2 ± 0.03	1.21 ± 0.08	97–105
Diabetic—3	11.29 ± 0.4	11.25 ± 0.2	98–101

^aUrine was prediluted 10X prior to the GOx-HRP assay. The urine further underwent 10X dilution, as the urinary sample volumes were 10% of the total assay volume. Therefore, the effective urine dilution during the GOx-HRP assay was 100X

^bUrine was not prediluted prior to the Ag-Pt nanozyme fabric assay. The urine underwent 10X dilution, as the urinary sample volumes were 10% of the total assay volume. Therefore, the effective urine dilution during the Ag-Pt nanozyme fabric assay was 10X

^cUrine glucose concentration was determined after considering the dilution factor of 100X

^dUrine glucose concentration was determined after considering the dilution factor of 10X

^eStandard deviation calculated from three independent experiments

^fRecovery calculated as (measured concentration/expected concentration) × 100

^gGlucose undetectable