Fig. 4.

¹H,¹⁵N chemical shift assignment of the imino groups of E. coli tRNA^Phe(GAA). (a) Sequence and L-shape 2D representation of modified E. coli tRNA^Phe(GAA). The same code is used as on Fig. 2. (b) (¹H,¹⁵N)-BEST-TROSY spectrum and assignment of the imino groups of unmodified and modified E. coli tRNA^Phe. Signals from the unmodified sample are represented in blue and signals from the modified sample are represented in red. Asterisks denote minor peaks in the unmodified tRNA^Phe corresponding to minor alternative folding conformations