FIG. 4.

Viability of normal human cells treated with virus. Normal human mammary epithelial cells, lung fibroblasts, or microvascular endothelial cells were cultured and infected with increasing concentrations of control virus ZZCB (squares), FTCB (circles), or FVCB (triangles) as indicated and assayed 3 days postinfection for cell viability via an MTS assay. Cell viability is plotted as the percent of untreated control cells ± standard deviations versus increasing concentrations of virus. Additional human mammary epithelial cells were plated and infected with 10⁹-P/ml concentrations of ZZCB, FTCB, or FVCB virus and were harvested 24 h postinfection. Equal amounts of total protein were loaded per well, and p53 protein was detected by Western analysis. A secondary Ab versus β-actin was used to verify equal loading between wells.