FIG. 6.
In vivo spread of p53 protein via VP22. SK HEP 1 cells were inoculated into the flanks of nude mice and allowed to establish for 10 days. A series of 5 daily intratumoral injections were then given at 5 × 109 of either control virus GFCB, FTCB, or FVCB per injection (n = 2 mice/group) as indicated. Tumors were excised 48 h after the last virus injection and processed for immunohistochemistry for p53 protein detection and for DNA extraction for PCR quantification of Ad hexon gene copies. Tumor sections from all six animals (identified by numbers) are shown along with the average copy number of hexon genomes for both animals per group per milligram of tissue (∗).