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. 2024 Oct 25;15:9237. doi: 10.1038/s41467-024-53545-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A UMAP plot of single-cell transcriptome data of small intestinal LPDC from Tph2^fl/fl and Tph2^fl/fl; Hand2-Cre mice. B Marker gene expression used for annotation of LPDC subsets. C Violin plots depicting the range of expression levels of Htr7 in distinct LPDC clusters. D Pie charts showing the proportion of pDC is decreased in Tph2^fl/fl; Hand2-Cre vs Tph2^fl/fl mice. E Comparison of the expression of the indicated genes in pDC between Tph2^fl/fl and Tph2^fl/fl; Hand2-Cre mice. The dot color reflects the level of gene expression, and the dot size represents the percentage of cells expressing the indicated gene. The genes labeled in red exhibit decreased expression in pDC from Tph2^fl/fl; Hand2-Cre mice and are consistent with the serotonin-induced genes observed in vitro. F GO Pathway analysis of genes that exhibited decreased expression in pDCs of Tph2^fl/fl; Hand2-Cre vs Tph2^fl/fl mice. G Violin plots depicting the range of expression levels of Ccr7 in distinct LPDC clusters. H Representative histogram plots showing the percentage of pDC (Live, CD45⁺, MHC-II⁺, CD64⁻, CD11c⁺, CCR7⁺) in LPDC from the small intestine of Tph2^fl/fl and Tph2^fl/fl; Hand2-Cre mice by flow cytometry. I Flow cytometry analyses of frequency of pDC in LPDC from the small intestine of Tph2^fl/fl and Tph2^fl/fl; Hand2-Cre mice. n = 6 mice per group. J Flow cytometry analyses of CD83 expression on pDC from the small intestine of Tph2^fl/fl and Tph2^fl/fl; Hand2-Cre mice. MFI mean fluorescence intensity. n = 6 mice per group. Data shown are means ± SD. Statistical analysis was performed by a two-tailed Mann–Whitney test in I and J.