Fig. 4. repNP vaccination protects WT but not TRIM21^−/− mice from liver pathology.

200X magnification of liver pathology from (a, b) WT sham vaccinated mice, (c, d) TRIM21^−/− sham vaccinated mice, (e, f) WT repNP vaccinated mice, and (g, h) TRIM21^−/− repNP vaccinated mice with (left) HE and (right) anti-CCHF IHC reactivity staining. Images are representative of groups of N = 6 mice. Liver samples from sham vaccinated and TRIM21^−/− repNP vaccinated mice show clusters of necrotic cellular debris multifocally dispersed throughout the hepatic plates. Sinusoidal mononuclear cells (Kupffer cells) and necrotic hepatocytes are immunoreactive. The liver samples from WT repNP vaccinated mice are normal.