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. 2024 Oct 25;7:1390. doi: 10.1038/s42003-024-07071-y

Fig. 2. Inhibition of AR activity reduces the differentiation of inflamed bPSC in organoid cultures.

Fig. 2

A Number of organoids formed with naïve, inflamed bPSC with or without 10 μM Enz treatment (Naïve groups, n = 10; Inflamed groups, n = 18). B Quantitation of nuclear AR staining in inflamed organoids treated with Enz (n = 12). C Diameter of organoids and (D) percentage of organoids with tubule-like structures in naïve, inflamed organoids or inflamed organoids treated with Enz (Naïve, n = 74; Inflamed, n = 47; Inflamed+Enz, n = 25). E Representative H&E images of naïve, inflamed organoids or inflamed organoids treated with Enz (Scale bar, 100 μm). F CK5 (green) and CK8 (red) staining showing lack of organization in Enz-treated inflamed organoids (Scale bar, 25 μm). G Enz significantly reduces the number of organoids formed with human PSC (control or Enz, n = 27). Data presented in this figure are mean ± SEM.