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. 2024 Oct 25;7:1390. doi: 10.1038/s42003-024-07071-y

Fig. 5. IL-1RA mediates inflammation-induced differentiation and AR activity in bPSC organoids.

Fig. 5

A Representative H&E images of naïve organoids treated with recombinant IL-1RA or neutralizing antibody (Ab) against IL-1α, showing an increase in stratification of treated organoids (Scale bar, 50 μm). B Organoid formation with naïve bPSC was significantly increased with recombinant IL-1RA (50 ng/mL) or IL-1α neutralizing Ab treatment (5 μg/mL) (Naïve_control, n = 12; Naïve_IL-1RA, n = 12; Naïve_IgG, n = 6; Naïve_IL-1α Ab, n = 9). C Quantitation of AR+ (both overall and nuclear) cells in naïve or inflamed organoids treated with IL-1RA (50 ng/mL) (All groups, n = 5). D AR staining (overall and nuclear) was increased in naïve organoids treated with an IL-1α neutralizing Ab (5 μg/mL) and decreased in inflamed organoids treated with an IL-1RA neutralizing Ab (5 μg/mL) (All groups, n = 5). E qRT-PCR showing that the AR target gene Steap4, which was induced in inflamed organoids and suppressed with Enz (left, n = 3), was upregulated with IL-1RA in naïve organoids and Enz treatment abolished the upregulation (right, n = 3). F The effects of IL-1RA or IL-1α neutralizing Ab were lost with naïve organoids derived from Ar_flox/y bPSC which were deprived of AR (All groups, n = 6). Data presented in this figure are mean ± SEM.