Table 1. Key specifications of common NGS throughput platforms.
| Throughput sequencing categories/Data output range (Gb: gigabyte and Tb: terabyte) | Common equipment/ Maximum Read Length (base pairs) /Maximum Raw data Output (Gb: gigabyte and Tb: terabyte) | Key applications |
|---|---|---|
| Low throughput (1–100 Gb) | iSeq 100-2x150 bp - 1.2 Gb
MiniSeq - 2x150 bp - 7.5 Gb DNBSEQ-E25-2x150 bp - 7.5 Gb Ion Torrent: Proton/PGM - 400 bp -10 Gb PacBio RS II - 60 kb - 10 Gb MiSeq - 2x300 bp - 15 Gb MinION - 100 kb - 50 Gb DNBSEQ-G99-2x300 bp - 96 Gb |
Targeted panel sequencing
Pathogen sequencing (microbes and viruses) 16S metagenomic sequencing |
| Medium throughput (100–1000 Gb) | NextSeq - 2x150 bp - 120 Gb
DNBSEQ-G50RS - 2x150 bp - 150 Gb GridION - 100 kb - 250 Gb PacBio Sequel II/IIe - 60 kb - 500 Gb |
Small whole-genome sequencing
Targeted panel sequencing (amplicon and gene panel) Gene expression profiling with mRNA-Seq miRNA and Small RNA analysis |
| High throughput (1000–10 000 Gb) | DNBSEQ-G400RS - 2 x 300 bp - 1.4 Tb
HiSeq - 2 x 150 bp - 1.5 Tb DNBSEQ-T7-2 x 150 bp - 7 Tb |
Whole-genome sequencing
Whole-exome sequencing Transcriptome sequencing Shotgun metagenomics |
| Ultra-high throughput (>10 Tb) | PromethION - 270 kb - 13.3 Tb
NovaSeq - 2x250 bp - 16 Tb DNBSEQ-T20×2RS - 2x150 bp - 72 Tb DNBSEQ-T10×4RS - 2x150 bp - 76.8 Tb |
Ultra-high-depth whole-genome sequencing of large genomes (Human, plant and animal)
Whole-exome sequencing Whole-transcriptome sequencing Methylation sequencing Shotgun metagenomics |