FIG. 9.

Effect of heparin on Ad infection of recombinant CHO cells. CHO K1 or A745 cells were transfected with an insertless vector (A) or with a vector encoding CAR (B). Ad2 (90 × 10⁹ virions) was preincubated with heparin (10 μg/ml) for 1 h at 37°C before being added to sparse CHO cells for 1 h at 4°C, and the cells were washed and incubated at 37°C for 72 h, as specified under Materials and Methods. Histograms show means of FFU from two separate experiments performed in triplicate.