Effect of storage time at 4 °C on the measurement of anti-HTLV-1 antibody quantitative data in CSF. Six cases of CSF—two low, two medium, and two high titers—were employed in the assay for each test kit (see the Section 2.5 for details). The CSF was stored at 4 °C for 0 h, 24 h, and 48 h, and the anti-HTLV-1 antibody titers were assessed. Measurements for each time point are displayed as the mean ± SD. A one-way repeated-measures ANOVA was used to compare the corresponding data among the three conditions. p ≤ 0.05 was considered statistically significant. COI, cutoff index; S/CO, signal-to-cutoff ratio. Information on each test kit is shown in Table 1.