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Detection of gene expression in liver tissue from LCMV-infected mice. Total RNA was isolated from liver tissues of LCMV-infected B6, Fas−/−, perf−/−, and gzmA×B−/− mice at 8 and 15 dpi, as well as from uninfected B6 mice. Specific mRNA for the indicated parameters was analyzed by RT-PCR as described in Materials and Methods.
