Figure 2.

Experimentally inoculated sheep and muntjac deer exhibited detectable viremia during the experimental time course. (A) Detection of BTV10 genomes by RT-qPCR in sheep and (B) muntjac deer. (C) Detection of BTV17 genomes by RT-qPCR in muntjac. Peripheral blood was drawn and processed for BTV detection. BTV was not detected in mock-inoculated controls at any time point. Assay used a threshold of 0.04 and the controls were valid. The data are represented as 1 over the Cycle threshold (Ct). Serum taken at blood draw time points was evaluated for detection of anti-BTV antibodies using a BTV-specific competitive ELISA (cELISA). (D) BTV10 in sheep, (E) BTV10 in muntjac, and (F) BTV17 in muntjac. A sample was considered positive if it was above 60% inhibition of the signal threshold.