FIG. 7.

Demonstration of replication compartment formation in D98-HR1 cells. Immunofluorescence assay performed on D98-HR1 cells that were transfected with wild-type Zta and incubated with BrdU 45 min prior to fixation. Cells were double stained for the polymerase processivity factor BMRF1 (a and d) using anti-BMRF1 monoclonal antibody and FITC-conjugated anti-mouse immunoglobulin secondary antibody and for BrdU (b and e) using anti-BrdU sheep antibody and rhodamine-conjugated anti-sheep immunoglobulin as the secondary antibody. Merge, overlays of the FITC and rhodamine images (c and f). Replication bodies are visible that double stain for BrdU incorporation and the presence of viral replication proteins, as exemplified by BMRF1.