Figure 6.

The overlapping acetylated proteins (A) and sites (B) between our PAO1 acetylome and the publicly available PA14 acetylomes. To start, the gene IDs provided by the two PA14 publications were converted to UniProt IDs in order to know which proteins to align in a later phase. Unfortunately, not every gene ID could be converted. As such, a few acetylated proteins were not included in this analysis. In total, 94.4% of Ouidir et al’s acetylated proteins and 94.9% of the sites were successfully mapped to PAO1. Moreover, 97.9% of Gaviard et al.’s acetylated proteins and 98.2% of their sites were successfully mapped to PAO1. Next, the overlap was determined after mapping the PA14 sites to their corresponding positions in PAO1 orthologs. Overall, we can see that many of the acetylated proteins and sites are unique to each dataset.