FIG. 2.
Digitized confocal optical sections of a time course of UL34 protein localization. HEp-2 cells were mock infected (A and B), infected at an MOI of 10 with UL34 null virus (vRR1072) for 10 h (C), or infected at an MOI of 10 with HSV-1(F) for 4 h (D to I), 6 h (J to L), 8 h (M to O), 10 h (P to R), or 20 h (S to X). All cells were fixed with formaldehyde, permeablized, and immunostained with chicken anti-UL34 antibody, detected with donkey anti-chicken Ig-Texas Red conjugate either alone (C) or with mouse anti-nucleoporin p62 detected with goat anti-mouse Ig-FITC conjugate (A, B, and D to X). Arrows indicate positions of invaginations and projections of the nuclear envelope (B and P to R) or membranous nuclear peripheral structures (V to X). Original magnifications: X630 (A to U); X1000 (V to X).