FIG. 3.
Digital confocal images of HEp-2 cells infected with wild-type HSV-1(F) at an MOI of 5. Cells were fixed and permeablized in ice-cold methanol at 0, 4, 8, 12, 16, and 20 h.p.i. Fixed cells were immunostained with UL31 rabbit antiserum and ICP4 monoclonal antibody. UL31-specific immunofluorescence was detected with a Texas Red-conjugated donkey anti-rabbit Ig antibody, and ICP4-specific immunofluorescence was detected with FITC-conjugated donkey anti-mouse Ig antibody. Original magnification, X600.