FIG. 5.
Digital confocal images of transfected HEp-2 cells fixed and permeabilized with ice-cold methanol at 18 h posttransfection. (A) Cells were transfected with UL31/pcDNA3 and probed with anti-UL31-GST antibody. (B) Nomarski DIC image of same microscopic field as in panel A. (C) Merge of images in A and B. (D) HEp-2 cells were transfected with UL34/pcDNA3 and stained with antiserum directed against UL34 protein. (E) Nomarski DIC image of same field as in panel D. (F) Merge of images in D and E. (G) Cells were cotransfected with UL31 and UL34 constructs and costained with UL31-specific and UL34-specific antisera. UL31-specific immunofluorescence is shown in panel G. (H) UL34-specific immunofluorescence in same cotransfected cells as in panel H. (I) Merge of UL31- and UL34-specific immunofluorescence, showing colocalization of transiently expressed UL31 and UL34 proteins as visualized by a yellow color upon merging of the separate images. (J) Nomarski DIC image of cotransfected cell shown in panels G to K. (K) Nomarski DIC image merged with the UL31- and UL34-specific immunofluorescence. Original magnification, X600.