FIG. 8.
Digital confocal images showing transiently tranfected HEp-2 cells. Cells were transfected with a UL34 expression construct (A to C) or a US3 expression construct (D to F) or cotransfected with UL34 and US3 expression constructs (G to I). At 24 h posttransfection cells were fixed with formaldehyde and immunostained with chicken anti-UL34 antibody detected with donkey anti-chicken Ig-Texas Red conjugate and rabbit anti-US3 detected with goat anti-rabbit Ig-FITC conjugate. Shown are confocal Z-sections. The high level of background UL34 staining seen in cells transfected with US3 alone (panels D to F) was not a regular feature of these experiments. Original magnification, X630.