FIGURE 1.

Evaluation of viability in PCLS. (A) Workflow for PCLS preparation and treatment. Viability analyzed with MTS assays for PCLS from (B) CDAHFD, (C) DEN, (D) CCl₄, and (E) TAA-induced cirrhosis rats. Experiments were independently repeated 3 times (n = 3). All values were expressed as the mean ± SEM with 2-tailed Student t tests or 1-way ANOVA analysis; ns indicates not significant. All treatment groups were statistically compared with the mock group. Abbreviations: CCl₄, carbon tetrachloride; CDAHFD, choline-deficient, l-amino acid–defined, high-fat diet; DEN, diethylnitrosamine; H&E, hematoxylin and eosin; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4 sulfophenyl)-2H-tetrazolium; PCLS, precision-cut liver slices; RT-qPCR, reverse transcription quantitative polymerase chain reaction; TAA, thioacetamide.