FIGURE 7.

The antifibrotic evaluation in human cirrhotic PCLS. (A) Quantitative RT-PCR analysis of mRNA expression of profibrogenic genes. Amount of collagen (B) measured with Sirius red staining and (C) quantified in PCLS from human cirrhosis treated with 5 μM erlotinib for 72 hours. (D) Heatmap of profibrogenic genes from 3 independently repeated experiments (n = 4). All values were expressed as the mean ± SEM with 2-tailed Student t tests. Significance is represented by *p < 0.05, **p < 0.01, ns indicates not significant. Abbreviations: PCLS, precision-cut liver slices; RT-PCR, reverse transcription polymerase chain reaction.