Table 1. Optimization of rice bran concentration and Incubation time.
| Bacterial strains | Incubation time (h) | Rice Bran % | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.5 | 1.0 | 1.5 | 2.0 | 2.5 | ||||||||||||
| CDM (g/L) | PHA (g/L) | PHA % | CDM (g/L) | PHA (g/L) | PHA % | CDM (g/L) | PHA (g/L) | PHA % | CDM (g/L) | PHA (g/L) | PHA % | CDM (g/L) | PHA (g/L) | PHA % | ||
| B. subtilis | 24 | 3.8 | 1.7 | 44.7 | 11.0 | 1.4 | 12.7 | 5.5 | 1.9 | 12.3 | 11.2 | 1.0 | 8.9 | 9.3 | 1.4 | 15 |
| 48 | 2.9 | 2.0 | 69 | 7.0 | 0.5 | 7.1 | 3.2 | 1.1 | 8.3 | 11.9 | 1.1 | 9.2 | 14.7 | 1.3 | 8.8 | |
| 72 | 2.6 | 0.7 | 26.9 | 2.5 | 0.002 | 8 | 6.4 | 1.6 | 9.7 | 11.4 | 0.5 | 4.4 | 10.0 | 0.5 | 5 | |
| 96 | 1.6 | 0.5 | 31.3 | 11.9 | 0.8 | 6.7 | 3.7 | 1.2 | 8.8 | 11.1 | 0.9 | 8.1 | 12.8 | 0.8 | 6.3 | |
| B. cereus | 24 | 4.5 | 1.6 | 35.5 | 6.3 | 1.3 | 20.6 | 8.7 | 1.0 | 11.5 | 10.1 | 1.0 | 9.9 | 5.6 | 0.6 | 10.7 |
| 48 | 6.7 | 0.6 | 8.9 | 4.8 | 1.1 | 23 | 9.3 | 0.7 | 7.5 | 13.0 | 0.9 | 6.9 | 8.9 | 1.2 | 13.5 | |
| 72 | 7.6 | 0.4 | 5.2 | 9.8 | 0.8 | 8.2 | 4.6 | 0.8 | 5.5 | 19.8 | 2.1 | 10.6 | 8.5 | 1.5 | 17.7 | |
| 96 | 4.6 | 1.1 | 23.9 | 11.0 | 2.2 | 20 | 11.5 | 2.2 | 19.1 | 16.6 | 2.5 | 15 | 11.4 | 1.6 | 14 | |
| Alcaligenes | 24 | 2.0 | 0.9 | 45 | 3.8 | 1.3 | 34.2 | 6.4 | 1.5 | 23.4 | 7.0 | 1.1 | 15.7 | 4.9 | 1.6 | 32.6 |
| 48 | 2.4 | 1.7 | 70.8 | 3.7 | 1.7 | 45.9 | 4.8 | 3.3 | 68.7 | 6.4 | 2.0 | 31.2 | 6.5 | 2.1 | 32.3 | |
| 72 | 3.1 | 2.7 | 87.1 | 5.0 | 3.2 | 64 | 6.4 | 3.0 | 46.8 | 14.5 | 3.3 | 22.7 | 7.3 | 3.4 | 46.5 | |
| 96 | 2.6 | 0.3 | 11.5 | 4.1 | 0.8 | 19.5 | 6.0 | 0.2 | 3.3 | 8.9 | 1.0 | 11.2 | 8.6 | 1.0 | 11.6 | |
| 120 | 3.3 | 0.4 | 12.1 | 6.5 | 0.7 | 10.8 | 9.8 | 0.9 | 9.2 | 10.0 | 0.9 | 9 | 10.6 | 1.8 | 16.9 | |
| P. aeruginosa | 24 | 2.9 | 1.0 | 34.4 | 2.6 | 1.1 | 42.3 | 5.1 | 1.2 | 23.5 | 5.3 | 1.1 | 20.7 | 6.9 | 1.1 | 15.9 |
| 48 | 2.5 | 1.9 | 76 | 3.9 | 2.9 | 74.3 | 4.3 | 1.7 | 39.5 | 5.4 | 1.0 | 18.5 | 15.5 | 2.9 | 18.7 | |
| 72 | 3.2 | 3.0 | 93.7 | 5.2 | 2.6 | 50 | 4.6 | 3.8 | 82.6 | 21.7 | 3.4 | 15.6 | 10.0 | 3.0 | 30 | |
| 96 | 1.9 | 0.5 | 26.3 | 3.7 | 0.6 | 16.2 | 3.9 | 0.8 | 20.5 | 9.1 | 1.1 | 12 | 6.7 | 0.7 | 10.4 | |
| 120 | 2.7 | 0.5 | 18.5 | 6.1 | 0.6 | 9.8 | 7.3 | 0.1 | 1.4 | 7.9 | 0.5 | 6.3 | 8.7 | 0.8 | 9.2 | |