FIG. 2.

Primary R5 HIV-1 isolates from advanced disease in the absence of X4 viremia retain selective cytopathicity for CCR5⁺ T cells. Experiments were performed with the indicated viruses (Table 1) as described in the legend to Fig. 1. (A) Depletion of CD4⁺ T cells, including total CD4⁺ T cells, and CCR5⁺ and CCR5⁻ subsets. (B) Depletion of memory and naive subsets of CD4⁺ T cells. (C) HIV-1 replication kinetics. Experiments with different donor specimens were conducted twice with dispersed cultures and once with tissue blocks; data from a representative experiment are presented.