(A) FACS analysis of CD45RO and CCR5 expression on unstimulated, cryopreserved CD4-positive lymphocytes from a healthy donor (HD1) and two HIV-1 recipients, Ams127 and ACH9012. PBMC samples were obtained 1 day (Ams127) and 4 months (ACH9012) after the moment of transmission. In three quadrants of each plot, the percentage of total CD4-positive lymphocytes is depicted. (B) FACS analysis of CD14 and CCR5 expression on unstimulated, cryopreserved monocytes from a healthy donor (HD1) and HIV-1 recipients Ams127 and ACH9012. PBMC samples were obtained 1 day (Ams127) and four and a half months (ACH9012) after transmission. In three quadrants of each plot, the percentage of total monocytes is depicted. The quadrants are set according to the immunoglobulin G isotype control for each blood donor in each experiment.