TABLE 1.

Genotypes and phenotypes of biological HIV-1 clones obtained from two parenteral donor-recipient pairs^a

^aD, donor; R, recipient; Env V3 no., envelope V3 sequence number; n, number of clones with the designated genotype and phenotype; ∗, clones isolated on MDM. Macrophagetropism of the clones was previously determined by infection of MDM obtained from two different blood donors for each of the biological clones (see references 27 and 30) and is depicted as the percentage of infected MDM cultures (−, 0%; +/−, 1 to 25%; +, 26 to 50%; ++, 51 to 75%; +++, 76 to 100%). R3, CCR3; X4, CXCR4; R5, CCR5. Blocking of replication of the biological clones was determined by infection of PHA-stimulated PBMCs in the absence or presence of RANTES, AMD3100, or SDF-1α. n (b), number of clones with the designated genotype and phenotype tested for blocking of replication on PBMCs; +, replication was blocked; −, replication was not blocked; +/−, replication of half of these clones was blocked. NT, not tested. 

^bResidual p24 production was observed for one biological virus clone with AMD3100 and for all biological virus clones inhibited by SDF-1α.