Fig. 5.

Analysis of mitochondrial respiration suggests that MT expression rescues the mtl-1 knock-out but does not display any additional impact of Cd²⁺ to mitochondrial respiration in the nematode model. The top diagrams (A) display the original oxygen consumption rate (OCR), without any (left) and with 12 µM Cd²⁺ concentration (right) in the medium during the measurement. Calculated spare capacities are presented in the column diagram below (B). Statistical analysis involved 3 individual experiments for each strain, each referenced to N2, with three wells per strain in each experiment and approximately 20 worms per well. Error bars indicate the standard deviation at 95% confidence. Exact worm numbers were determined after the experiment. Significance is marked by asterisks.