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. 2024 Oct 26;14:25474. doi: 10.1038/s41598-024-76601-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Quantitative analysis of metabolic fraction-treated flies. (a) Phenotypic score determined through quantitative analyses using the Flynotyper plugin on ImageJ. None of the treatment groups presented significant differences compared with the control group (vehicle) (p > 0.5) n ≥ 3. (b) Total vacuole area. Compared with control flies, only flies treated with the EtOAc fraction presented a reduced total area of vacuoles (p = 0.023) n = 3. (c) Retinal thickness. None of the treatment groups presented significant differences compared with the control group (vehicle) (p > 0.5) n = 3. (d) Relative amyloid quantification through ThT assay; only flies treated with the EtOAc fraction presented a greater amyloid content than did the control group (p < 0.0001) triplicate of of pool with 10 heads in each. The data are shown as individual values, the mean ± S.E.M. (unpaired t test).