Skip to main content
. Author manuscript; available in PMC: 2024 Oct 27.
Published in final edited form as: Neurobiol Dis. 2024 Aug 24;200:106647. doi: 10.1016/j.nbd.2024.106647

Fig. 5.

Fig. 5.

Progressive α-syn pathology (See data availability for open access to all analysis results and additional comparisons). A–J: mice injected with PFFs at 4 weeks of age (2- and 4-months post-injection). Bubble plots - 1 marker/ROI, counts vs ROI area grouped by brain region between post-injection, 2 month (2 m) and 4 month (4 m) time points across injection types, PFF (red markers) and PBS (blue markers), within the En1+/− genotype. Histograms - 1 mouse/marker, bars = treatment group means, error bars ± SEM. 2 m En1/SYN n = 7, 4 m En1/SYN n = 9. B) Striatum: Significant increases in mean counts of Lewy body-like inclusions between time points within treatment group (p < 0.001, En1/SYN vs WT/PBS p < 0.001). D) Motor cortex: Significant increases in mean counts of Lewy body-like inclusions between time points within treatment group (p < 0.001, En1/SYN vs WT/PBS p = 0.001). F) Cingulate cortex: Significant increases in mean counts of Lewy body-like inclusions between time points within treatment group (p < 0.001, En1/SYN vs WT/PBS p = 0.042). H) Amygdala: No significant phenotype validation relevant increases in mean counts of Lewy body-like inclusions between time points within treatment group (En1/SYN vs WT/PBS p > 0.05). J) Substantia Nigra: No significant phenotype validation relevant increases in mean counts of Lewy body-like inclusions between time points within treatment group (En1/SYN vs WT/PBS p > 0.05). K–T: mice injected with PFFs at 8 weeks of age (6 months post-injection). Bubble plots - 1 marker/ROI, counts vs ROI area grouped by brain region. Treatment color coding: En1/PBS in orange, En1/SYN = red, WT/PBS = blue, WT/SYN = green. Histograms - 1 mouse/marker, bars = treatment group means, error bars ± SEM. Markers with white fill = PFF and black fill = PBS, circle markers = Engrailed1 and triangle = WT. See data availability for access to all analysis results. Significant increases in mean counts of Lewy body-like inclusions between treatment groups in all observed brain regions. L) Striatum (p < 0.0001, En1/SYN vs WT/PBS p < 0.0001). N) Motor cortex (p < 0.0001, En1/SYN vs WT/PBS p = 0.0002). P) Cingulate cortex (p < 0.0001, En1/SYN vs WT/PBS p = 0.0032). R) Amygdala (p < 0.0001, En1/SYN vs WT/PBS p = 0.0003). T) Substantia Nigra (p < 0.0001, En1/SYN vs WT/PBS p = 0.0032).