Figure 1.

Temporal expression of HO-1, CD206, and CD68 in MG after ischemic stroke. Male C57BL/6 mice were subjected to sham (n=3/per time point of day 1-6) or 40 min MCAO (n=10/per time point of day 1-5; n=6/day 6). On day 1, 2, 3, 5, and 6 post-injury, mice were sacrificed, and the ischemic brains were harvested. The contralateral (Contra.) and ipsilateral (Ipsi.) hemispheres of sham and MCAO mice were subjected to mononuclear cell isolation. The isolated cells were stained with antibodies against CD45 and CD11b followed by intracellular staining with HO-1, CD206, or CD68 to assess CD45^intCD11b⁺ MG positive for (A) HO-1, (B) CD206, or (C) CD68 expression, respectively. Statistical analysis was comparing the ipsilateral hemisphere of MCAO mice to that of sham controls. *p<0.05; **p<0.01; ***p<0.001 by unpaired t test or Mann-Whitney U test.