FIG. 2.

JSRV and amphotropic vectors show similar levels of sensitivity to inactivation by ES 7X cleaning solution. HT-1080 cells were plated at 7.5 × 10⁴ cells per 3.5-cm-diameter well of six-well plates. One day later, the cells were fed 2 ml of medium containing 4 μg of Polybrene per ml. LAPSN(PJ4) (A) and LAPSN(PA317) (B) vectors were treated with ES 7X at the indicated concentrations for 10 s or 1 min, and 1-, 5-, and 10-μl samples of the treated vectors were then added to the cells. At these dilutions, the ES 7X had no apparent effect on the health or growth rate of the cells. Two days after exposure to the vectors, the cells were fixed and stained for alkaline phosphatase-positive foci. Means and standard deviations from three to four experiments are shown. Data points on the x axes represent values below the limit of detection: <0.4% for the JSRV vector and <0.14% for the amphotropic vector.