TABLE 2.

JSRV and amphotropic vectors show similar levels of sensitivity to ethanol inactivation^a

Ethanol concn (%)	JSRV vector inactivation (%) after incubation with ethanol for:		Amphotropic vector inactivation (%) after incubation with ethanol for:
	10 s	1 min	10 s	1 min
0	0	0	0	0
10	38 ± 14	56 ± 11	39 ± 39	62 ± 16
30	99.1 ± 0.8	>99.8	99.3 ± 0.9	>99.9
50	>99.7	>99.8	>99.9	>99.9
70	>99.7	>99.7	>99.9	>99.9

^aHT-1080 cells were plated at 7.5 × 10⁴ cells per 3.5-cm-diameter well in six-well plates on day 1. On day 2, the medium was replaced with 2 ml of medium containing 4 μg of Polybrene per ml. JSRV [LAPSN(PJ4)] and amphotropic [LAPSN(PA317)] vectors were treated with ethanol at the indicated concentrations, and 1-, 5-, and 10-μl samples of the treated vectors were then added to the cells either 10 s or 1 min after ethanol addition. At this dilution, the ethanol had no apparent effect on the health or growth rate of the cells. Two days after exposure to the vectors, the cells were fixed and stained for alkaline-phosphatase-positive foci. Means and standard deviations from three to four experiments are shown.