FIG. 3.

Autoradiographic image of RPA of levels of total human cyclin RNA isolated from quiescent HEL fibroblast cells induced with 10% fetal bovine serum and mock infected (lanes 3 to 9) or quiescent HEL fibroblasts infected with HSV-1(F) (lanes 10 to 15) or R7914 (lanes 16 to 21). Total RNAs from HEL fibroblasts harvested at the times indicated were annealed to the radiolabeled cyclin probe set, and then unprotected RNA was digested with RNase. The labeled probe protected by hybridization with complementary RNA was electrophoretically separated on 5% nondenaturing polyacrylamide gels and detected by autoradiography. The cyclin species are indicated between lanes 2 and 3.