TABLE 1.
Quantification of protected cyclin RNA speciesa
| RNA species | Virus | Radioactivity at indicated time (h) after initiation of infection
|
|||||
|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 4 | 8 | 16 | ||
| Cyclin A | Mock | 1.00 | 1.25 | 0.63 | 0.38 | 0.75 | 2.88 |
| HSV-1(F) | 1.00 | 1.00 | 0.88 | 0.75 | 0.80 | 0.00 | |
| R7914 | 1.00 | 0.88 | 1.13 | 1.13 | 1.00 | 0.00 | |
| Cyclin B | Mock | 1.00 | 1.32 | 0.96 | 0.56 | 0.68 | 1.24 |
| HSV-1(F) | 1.00 | 1.16 | 0.84 | 0.72 | 0.52 | 0.00 | |
| R7914 | 1.00 | 1.36 | 1.04 | 0.76 | 0.52 | 0.00 | |
| Cyclin D1 | Mock | 1.00 | 0.97 | 1.30 | 1.16 | 1.58 | 1.29 |
| HSV-1(F) | 1.00 | 0.83 | 0.79 | 0.60 | 0.33 | 0.42 | |
| R7914 | 1.00 | 0.78 | 0.68 | 0.45 | 0.37 | 0.36 | |
| Cyclin D2 | Mock | 1.00 | 0.97 | 1.22 | 0.97 | 1.21 | 1.09 |
| HSV-1(F) | 1.00 | 0.90 | 0.88 | 0.76 | 0.49 | 0.39 | |
| R7914 | 1.00 | 0.90 | 0.71 | 0.67 | 0.56 | 0.49 | |
| Cyclin D3 | Mock | 1.00 | 0.92 | 1.37 | 1.22 | 1.58 | 1.54 |
| HSV-1(F) | 1.00 | 0.74 | 1.10 | 0.62 | 0.57 | 0.15 | |
| R7914 | 1.00 | 0.73 | 0.81 | 0.59 | 0.31 | 0.12 | |
| Cyclin A1 | Mock | 1.00 | 1.22 | 1.14 | 1.25 | 1.41 | 1.30 |
| HSV-1(F) | 1.00 | 0.56 | 0.65 | 0.47 | 0.45 | 0.00 | |
| R7914 | 1.00 | 0.54 | 0.37 | 0.35 | 0.30 | 0.00 | |
a
Quantification was performed on protected species in total RNA isolated from quiescent HEL cells induced with 10% fetal bovine serum or mock infected or infected as described in Results. Radioactivity was quantified with the aid of a Storm 860 phosphorimager and ImageQuant software and normalized to amounts detected at time zero (beginning of the experiment).