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. 1991 Sep 1;278(Pt 2):365–368. doi: 10.1042/bj2780365

Synthesis of a gene for rat liver fatty-acid-binding protein and its expression in Escherichia coli.

A F Worrall 1, C Evans 1, D C Wilton 1
PMCID: PMC1151350  PMID: 1898328

Abstract

A gene coding for rat liver fatty-acid-binding protein (FABP) has been constructed by the single-step ligation of ten synthetic oligonucleotides. The gene has been cloned into the bacterial expression vector pKK223-3. Induction of protein synthesis from this gene results in over expression of an FABP that is indistinguishable in its structure and binding properties with that isolated from rat liver.

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Selected References

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