REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
4′,6-diamidino-2-phenylindole	Sigma	D9542
Ethanol	Commercial Alcohols	P016EAAN
Iodoacetamide	Sigma	I6125
Ammonium bicarbonate	BioShop	AMC107
Tris(2-carboxyethyl) phosphine	Sigma	C4706
n-dodecyl-β-D-maltoside	Sigma	850520P
Invitrosol	Thermo Fisher Scientific	MS10007
Experimental models: Cell lines
Human: MCF10A	ATCC	CRL-10317
Software and algorithms
MSFragger (v.3.8) run using FragPipe (v.20.0)	Kong et al.9 and Teo et al.10	N/A
ProteinProphet	Nesvizhskii et al.11	N/A
Percolator	Kall et al.12	N/A
IonQuant v.1.9.8	Yu et al.13	N/A
Philosopher v.5.0.0	da Veiga Leprevost et al.14	N/A
Other
Orbitrap Fusion Tribrid mass spectrometer	Thermo Scientific	N/A
EASY-nLC 1000 HPLC system	Thermo Scientific	N/A
Acclaim PepMap 100 C18 HPLC column	Thermo Scientific	164946
EASY-Spray HPLC column	Thermo Scientific	ES903
Teflon-coated microscope slides	Tekdon (https://tekdon.com)	Custom order. Details in File S1
Microscrope slide holder	This study	Template provided as File S2 and S3. Available g-code at NIH 3D: 3DPX-020483
BD FACSAria fusion flow cytometer (or equivalent plate-compatible instrument)	BD Biosciences	N/A