Lysis buffer (freshly prepare, do not store)

Reagent	Final concentration	Amount
1 M Ammonium Bicarbonate	100 mM	10 μL
Acetonitrile (MS grade)	15%	15 μL
Invitrosol (5 $\times$)	0.5 $\times$	10 μL
2% n-dodecyl-β-D-maltoside	0.06%	3 μL
100 mM Tris(2-carboxyethyl) phosphine	5 mM	5 μL
H2O (MS grade)	N/A	57 μL
Total	N/A	100 μL