Digest buffer (freshly prepare, do not store)
| Reagent | Final concentration | Amount |
|---|---|---|
| 250 mM Iodoacetamide | 35 mM | 14 μL |
| Trypsin/Lys-C (MS grade) | variable | variable |
| H2O (MS grade) | N/A | To 100 μL |
| Total | N/A | 100 μL |
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