Figure 4.

SBRT/IL-12mRNA treatment remodels the CD4 and CD8 T cell response in tumors

(A and B) Subclustered UMAPs and relative proportions of CD4 and CD8 T cell subpopulations following each treatment. (C and D) Ratios of activated/cycling to exhausted CD4 and CD8 T cells. (E) Ratios of activated/cycling to regulatory CD4 T cells. (F and G) Co-expression heatmaps for Mki67/Lag3 for CD4 T cells and Prf1/Ifng for CD8 T cells for each treatment. (H and I) Trajectory analysis for CD4 and CD8 T cells with Naive T cells utilized as the root population in each case. Black circles indicated branch nodes and white circles signify potential terminal outcomes along the trajectory. See materials and methods for further details.