Skip to main content
. 2024 Sep 30;35(4):102350. doi: 10.1016/j.omtn.2024.102350

Figure 7.

Figure 7

IFNγ expression is induced by SBRT/IL-12mRNA and is essential to treatment efficacy

(A) Tumor IFNγ concentration measured by luminex at 24 h and 96 h post IL-12mRNA injection. (B) IFNγ+ CD4 and CD8 T cells at 24 h and 72 h post IL-12mRNA injection. (C) UMAPs for CD4 and CD8 T cells for each treatment group depicting levels of IFNγ gene expression. (D) SBRT/IL-12mRNA efficacy in IFNγ KO vs. wild-type mice. (E) Proliferation and frequency of CD4 and CD8 T cells in IFNγ KO vs. wild-type mice following SBRT/IL-12mRNA treatment. n = 5/group from two independent experiments in (A). n = 10/group from two independent experiments. n = 5/group in (D) and (E). One-way ANOVA followed by Tukey’s test. Significance against untreated and between treatments are reported. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001. Lines represent mean ± SEM for (A), (B), (D) and (E).