FIG. 2.

Localization of RAF-2p48 in influenza virus-infected cells. HeLa cells were infected with influenza A/PR/8/34 virus at a multiplicity of 10 (G to L) or mock infected (A to F). At 9 h postinfection, cells were fixed with PBS containing 3% paraformaldehyde and an indirect immunofluorescence assay was carried out as described in Materials and Methods. RAF-2p48 (A, D, G, and J) and SC35 (B, E, H, and K) were detected with rhodamine- and FITC-conjugated secondary antibodies, respectively. Images stained with rhodamine and FITC are merged (C, F, I, and L). Magnification is indicated by scale bars.