TABLE 1.
Molecular masses of lysyl endopeptidase-digested peptides from RAF-2p48 and amino acid sequences corresponding to the lysyl endopeptidase-digested peptide molecular mass database
| No. | Predicted amino acid sequence from database | Mass (Da)
|
||
|---|---|---|---|---|
| Observed | Theoretical | Difference | ||
| 1 | EYERFSK | 958.48 | 958.46 | +0.02 |
| 2 | EIRPVCRK | 1,058.61 | 1,058.58 | +0.03 |
| 3 | VAVFFGGLSIK | 1,137.69 | 1,137.67 | +0.02 |
| 4 | HFILDECDK | 1,177.55 | 1,177.52 | +0.03 |
| 5 | LTLHGLQQYYVK | 1,462.85 | 1,462.81 | +0.04 |
| 6 | GLAITFVSDENDAK | 1,479.77 | 1,479.73 | +0.04 |
| 7 | FMQDPMEIFVDDETK | 1,844.67 | 1,844.81 | −0.14 |
| 8 | FMQDPMEIFVDDETK…1Met-Ox | 1,860.66 | 1,860.81 | −0.15 |
| 9 | FMQDPMEIFVDDETK…2Met-Ox | 1,876.70 | 1,876.81 | −0.11 |
| 10 | NCPHIVVGTPGRILALARNK | 2,187.25 | 2,187.22 | +0.03 |
| 11 | KNCPHIVVGTPGRILALARNK | 2,315.35 | 2,315.31 | +0.04 |
| 12 | MLEQLDMRRDVQEIFRMTPHEK…1Met-Ox | 2,818.41 | 2,818.39 | +0.02 |
| 13 | MLEQLDMRRDVQEIFRMTPHEK…2Met-Ox | 2,834.38 | 2,834.39 | −0.01 |
| 14 | Ac-AENDVDNELLDYEDDEVETAAGGDGAEAPAKa | 3,264.45 | 3,264.37 | +0.08 |
a
The amino terminus of p48 was acetylated alanine (position 14).