TABLE 6.
Compositional data, intake values (calculated for chickens for fattening at 15 mg/kg complete feed), reference points and margin of exposure (MOE) for the individual components of Spanish type origanum oil classified according to assessment groups, and combined margin of exposure (MOET) for each assessment group.
| Essential oil composition | Exposure | Hazard characterisation | Risk characterisation | |||||
|---|---|---|---|---|---|---|---|---|
| Assessment group | FLAVIS‐No | Highest conc. in the oil | Highest feed conc. | Intake a | Cramer Class b | NOAEL c | MOE d | MOET e |
| Constituent | – | % | mg/kg | mg/kg bw per day | – | mg/kg bw per day | – | – |
| CG 1 | ||||||||
| Methyl citronellate | 09.517 | 0.01 | 0.0017 | 0.00015 | I | 3 | 20,253 | |
| CG 4 | ||||||||
| Lavandulyl acetate | 09.612 | 0.06 | 0.0083 | 0.00074 | I | 3 | 4051 | |
| CG 5 | ||||||||
| Oct‐1‐en‐3‐ol | 02.023 | 0.34 | 0.0503 | 0.00451 | (I) | 6.7 | 1485 | |
| Octan‐3‐one | 07.062 | 0.20 | 0.0296 | 0.00265 | II | 0.91 | 343 | |
| Octan‐3‐ol | 02.098 | 0.03 | 0.0047 | 0.00042 | I | 3 | 7187 | |
| MOET CG 5 | 268 | |||||||
| CG 6 | ||||||||
| Linalool | 02.042 | 1.34 | 0.2004 | 0.01799 | (I) | 117 | 6503 | |
| 4‐Terpinenol | 02.072 | 0.82 | 0.1230 | 0.01104 | (I) | 125 f | 11,320 | |
| 2‐(4‐Methylphenyl)propan‐2‐ol | 02.042 | 0.03 | 0.0041 | 0.00039 | I | 3 | 7682 | |
| trans‐p‐2‐Menthen‐1‐ol | – | 0.02 | 0.0035 | 0.00031 | I | 3 | 9686 | |
| Spathulenol | – | 0.02 | 0.0027 | 0.00024 | I | 3 | 12,377 | |
| MOET CG 06 | 1798 | |||||||
| CG 8 | ||||||||
| d,l‐Borneol | 02.016 | 0.46 | 0.0693 | 0.00622 | (I) | 15 | 2411 | |
| Camphor | – | 0.22 | 0.0329 | 0.00295 | II | 0.91 | 309 | |
| Dihydrocarvone | 07.128 | 0.04 | 0.0062 | 0.00055 | II | 0.91 | 1648 | |
| Carvenone | – | 0.02 | 0.0029 | 0.00026 | II | 0.91 | 3557 | |
| MOET CG 08 | 220 | |||||||
| CG 10 | ||||||||
| 2‐Methyl‐5‐(propan‐2‐ylidene)cyclohexane‐1,4‐diol | – | 0.12 | 0.0183 | 0.00164 | I | 3 | 1826 | |
| CG 13 | ||||||||
| Linalool oxide | 13.140 | 0.01 | 0.0021 | 0.00019 | II | 0.91 | 4827 | |
| CG 16 | ||||||||
| 1,8‐Cineole | 03.001 | 0.28 | 0.0422 | 0.00378 | (II) | 100 | 26,428 | |
| CG 25 | ||||||||
| Thymohydroquinone | – | 0.20 | 0.0300 | 0.00269 | I | 3 | 1114 | |
| CG 26 | ||||||||
| 1‐Isopropyl‐2‐methoxy‐4‐methylbenzene | 04.043 | 0.25 | 0.0375 | 0.00337 | I | 3 | 891 | |
| CG 31, II (Acyclic alkanes) | ||||||||
| Myrcene | 01.008 | 1.25 | 0.1869 | 0.01678 | (I) | 44 | 2622 | |
| CG 31, III (Cyclohexene hydrocarbons) | ||||||||
| γ‐Terpinene | 01.020 | 5.72 | 0.8580 | 0.07703 | (I) | 250 | 3246 | |
| α‐Terpinene | 01.019 | 1.45 | 0.2178 | 0.01955 | (I) | 30 g | 1534 | |
| d‐Limonene | 01.045 | 0.39 | 0.0588 | 0.00528 | (I) | 250 | 47,361 | |
| α‐Phellandrene | 01.006 | 0.26 | 0.0393 | 0.00353 | (I) | 30 g | 8503 | |
| MOET CG 31, III | 910 | |||||||
| CG 31, IVe (Benzene hydrocarbons, alkyl) | ||||||||
| p‐Cymene | 01.002 | 8.28 | 1.2425 | 0.11154 | (I) | 154 | 1381 | |
| Abietatriene | – | 0.03 | 0.0047 | 0.00044 | I | 3 | 7187 | |
| MOET CG 31, IVe | 1158 | |||||||
| CG 31, V (Bi‐, tricyclic, non‐aromatic hydrocarbons) | ||||||||
| β‐Caryophyllene | 01.007 | 3.69 | 0.5540 | 0.04973 | (I) | 222 | 4464 | |
| α‐Thujene | – | 0.88 | 0.1322 | 0.01186 | (I) | 222 | 18,713 | |
| α‐Pinene | 01.004 | 0.88 | 0.1322 | 0.01186 | (I) | 222 | 18,713 | |
| Camphene | 01.009 | 0.15 | 0.0224 | 0.00201 | I | 3 | 1495 | |
| Palustradiene | – | 0.06 | 0.0092 | 0.00082 | I | 3 | 3652 | |
| MOET CG 31, V | 785 | |||||||
| CG 32 | ||||||||
| β‐Caryophyllene epoxide | 16.043 | 0.57 | 0.0852 | 0.00765 | (III) | 119 | 15,558 | |
| Thujones | ||||||||
| α‐Thujone | – | 0.19 | 0.0287 | 0.00257 | (III) | 8 | 3110 | |
| β‐Thujone | – | 0.03 | 0.0047 | 0.00042 | (III) | 8 | 19,164 | |
| 2676 | ||||||||
Intake calculations for the individual components are based on the use level of 15 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight.
When a NOAEL value is available or read‐across is applied, the allocation to the Cramer class is put into parentheses.
Values in bold refer to those components for which the NOAEL value was available, values in italics are the 5th percentile of the distribution of NOAELs of the corresponding Cramer Class, other values (plain text) are NOAELs extrapolated by using read‐across.
The MOE for each component is calculated as the ratio of the reference point (no observed adverse effect level, NOAEL) to the intake.
The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal of the sum of the reciprocals of the MOE of the individual substances.
An uncertainty factor of 2 was applied to the NOAEL of 250 mg/kg bw per day for terpineol (short duration of the study).
An uncertainty factor of 2 was applied to the NOAEL of 60 mg/kg bw per day for α‐terpinene (nature of the study).