Fig. 2. Preparation and characterization of AdECM derived from bovine adipose tissue. (a) Structural change of bovine adipose tissues after decellularization. (b) Histological sections of native and decellularized tissue. The inserts showed immunofluorescence images of DAPI staining (nuclei). (c) The content of collagen, GAGs, and DNA were quantified in native adipose tissue (native tissue) and decellularized adipose tissues (AdECM). *P < 0.05, compared to native tissue. (d) The ability of gelation was examined in solution of 0.5, 0.75, and 1% AdECM/0.5 M acetic acid. (e and f) Rheological properties of 0.5, 0.75, and 1% AdECM during gelation. (g) Cryo-SEM images of AdECM exhibited the network of fiber after gelling. Scale bar = 2 μm. Three batches of bovine adipose tissue were processed for the preparation of AdECM.