Fig. 6. A 3D-printed microfluidic device sustained vasculogenesis. (a) Illustration of the 3D-printed microfluidic device and experimental setup. (b) Top view of the microfluidic device. (c) Schematic showing the dimensions of the 3D-printed microfluidic device. (d) Experimental procedures for constructing vasculature in a microfluidic device. (e) Vessels cultured in a cultured plate or in a microfluidic device (dynamic) for 14 days, stained with F-actin (red). Scale bar = 100 μm. (f and g) Quantification of vessel length and diameter using ImageJ. *P < 0.05, versus vessels embedded in fibrin matrix without 0.5% AdECM in the cultured plate. ^#P < 0.05, compared to vessels cultivated in an AdECM-containing fibrin gel on a culture plate by one-way ANOVA. (h) VEGF content in media from the cultivation of vasculature for 10 to 14 days was measured. *P < 0.05, compared to the content of VEGF in media after cultivating vessels on a cultured plate for 14 days by one-way ANOVA. Results are presented as the mean percentage (±SD) of three independent experiments.