Fig. 7.

Stimulated BMMC release numerous cytokines and chemokines. Cytokine/chemokine secretion was analyzed for (A) RBL-CCR1 cells and (B) 6 weeks-cultured BMMC. Each condition was tested using 40 000 cells in triplicate wells, overnight sensitized and/or stimulated with the appropriate ligands for 30 min at 37°C. Supernatants were collected and stored/transported immediately at −20°C until analyzed. Five independent experiments were conducted. Of these, three randomly selected experiments were compared for statistical analysis. Error bars represent SEM, and statistically significant differences were identified using analysis of variance, *P < 0.05. Only the samples showing significant differences compared with co-stimulation are marked.