Figure 3.

Deletion of Nf2 and Sav1 or WWC1–3 in the mesothelium of pleural cavities in mice led to mesothelioma development

(A) Illustration of intrathoracic injection of Adeno-Cre in mice.

(B) Schematic diagram indicating the experimental procedure. Mice (n = 5 for each group) at 4-week-old were injected with Adeno-Cre to delete corresponding genes. Lungs and hearts were collected after 4 weeks for histological analysis.

(C) Gross images and histological analysis of lungs and hearts. H&E and IHC staining for WT1, Mesothelin, and Ki67 expression were performed. Genotypes of mice were indicated. Scale bars, 5 mm for gross lung and heart images and 50 μm for H&E or IHC images.

(D and E) Quantification of the average thickness of lung (D) and heart (E) mesothelium in mice with different genotypes. Mesothelium thickness is primarily defined by Mesothelin staining signals.

(F) Survival curves of mice with different genotypes following Adeno-Cre injection. The number of mice used was indicated.

Data are presented as mean ± SEM. Statistical significance: ∗, p < 0.05, ∗∗, p < 0.01, ∗∗∗, p < 0.001. The Student’s t test was used for statistical analysis.

See also Figure S3.