REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-Merlin (D3S3W)	Cell Signaling Technology	Cat#12888S; RRID: AB_2650551
Rabbit monoclonal anti-LATS1(C66B5)	Cell Signaling Technology	Cat#3477S; RRID: AB_2133513
Rabbit monoclonal anti-LATS2(D83D6)	Cell Signaling Technology	Cat#5888S; RRID: AB_10835233
Rabbit monoclonal anti-SAV1(D6M6X)	Cell Signaling Technology	Cat#13301S; RRID: AB_2798176
Mouse monoclonal anti-WWC1	Zhang et al.74	N/A
Rabbit polyclonal anti-WWC2	Abcam	Cat#ab126356; RRID: AB_11140331
Rabbit polyclonal anti-WWC3	Qi et al.30	N/A
Rabbit monoclonal anti-YAP(D8H1X)	Cell Signaling Technology	Cat#14074S; RRID: AB_2650491
Rabbit monoclonal anti-Vinculin(E1E9V)	Cell Signaling Technology	Cat#13901S; RRID: AB_2728768
Rabbit monoclonal anti-Phospho-YAP(Ser127)	Cell Signaling Technology	Cat#13008; RRID: AB_2650553
Rabbit monoclonal anti-Non-phospho(Active) YAP(Ser127)(E6U8Z)	Cell Signaling Technology	Cat#29495; RRID: AB_2798974
Rabbit polyclonal anti-CYR61(H-78)	Santa Cruz Biotechnology	Cat#sc-13100; RRID: AB_2088733
Rabbit polyclonal anti-Ki67	Abcam	Cat#ab15580; RRID: AB_443209
Mouse monoclonal anti-YAP1(63.7)	Santa Cruz Biotechnology	Cat#sc-101199; RRID: AB_1131430
Rabbit monoclonal anti-WT1	Cell Signaling Technology	Cat#83535; RRID: AB_2800020
Rabbit polyclonal anti-Mesothelin	Thermo Fisher Scientific	Cat#PA5-79698; RRID: AB_2746813
Rabbit monoclonal anti-Flag tag (D6W5B)	Cell Signaling Technology	Cat#14793S; RRID: AB_2572291
Mouse monoclonal anti-FLAG M2(HRP conjugated)	Sigma-Aldrich	Cat#A8592; RRID: AB_439702
Rabbit monoclonal anti-Phospho-LATS1(Thr1079)(D57D3)	Cell Signaling Technology	Cat#8654S; RRID: AB_10971635
Mouse monoclonal anti-Myc Tag	MBL International	Cat#M192-3; RRID: AB_11160947
Mouse monoclonal anti-GAPDH(HRP conjugated)	Abways Technology	Cat#AB2000
Goat polyclonal anti-CTGF(L-20)	Santa Cruz Biotechnology	Cat#sc-14939; RRID: AB_638805
Goat anti-rabbit IgG antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat#A11001; RRID: AB_2534069
Bacterial and virus strains
Trans5α Chemically Competent Cell	TransGen Biotech	Cat#CD201-01
pLVX lentivirus	This paper	N/A
LentiCRISPR v2	Sanjana et al.75	Addgene 52961
Adeno-Cre	HANBIO	Cat#HBAD-1010
Adeno-associated virus (AAV)	This paper	N/A
Chemicals, peptides, and recombinant proteins
DAPI	Sigma-Aldrich	Cat#D9542
Fetal bovine serum	Invitrogen	Cat#10091-148
Protease inhibitor cocktail	MCE	Cat#HY-K0010
Phosphatase inhibitor Cocktail I	MCE	Cat#HY-K0021
Phosphatase inhibitor Cocktail II	MCE	Cat#HY-K0022
Phosphatase inhibitor Cocktail II	MCE	Cat#HY-K0022
PEI	Polysciences	Cat#23966-2
PolyJet	Signagen Laboratories	Cat#SL100688
Envision anti-Rabbit	DAKO	Cat#K4002
DAB reagent	GeneTech	GK500705
Prime STAR Max DNA polymerase	Takara	Cat#R045A
VT103	Selleck	Cat#E1598
VT107	Selleck	Cat#E1599
Critical commercial assays
TaKaRa MiniBEST Universal RNA Extraction Kit	Takara	Cat#9767
TB Green® Premix Ex Taq™ (Tli RNaseH Plus)	Takara	Cat#RR420A
TransScript® First-Strand cDNA Synthesis SuperMix	TransGen Biotech	Cat#AT301-03
ClonExpress MultiS One Step Cloning Kit	Vazyme	Cat#C113-02
High-sig ECL Western Blotting	Tanon	Car#180-501
Cell Counting Kit(CCK-8)	YEASEN	Cat#40203ES60
Deposited data
TCGA Mesothelioma data	Hmeljak et al.42	https://portal.gdc.cancer.gov/projects/TCGA-MESO, dbGap Study Accession:phs000178.v11.p8
Experimental models: Cell lines
Human: HEK293A	Yu et al.76	N/A
Human: HEK293T	National collection of Authenticated Cell Cultures	Cat#SCSP-502
Human: NCI-H2452	Tang et al.51	N/A
Human: NCI-H2373	Tang et al.51	N/A
Human: NCI-H2052	Tang et al.51	N/A
HEK293A NF2 KO	Qi et al.30	N/A
Experimental models: Organisms/strains
Mouse: Nf2fl/fl (C57/BL6J background)	Wang et al.77	N/A
Mouse: Savfl/fl(C57/BL6J background)	Ji et al.78	N/A
Mouse: Wwc1fl/fl;Wwc2fl/fl (C57/BL6J background)	Qi et al.31	N/A
Mouse: Nf2fl/fl;Savfl/fl (C57/BL6J background)	Qi et al.31	N/A
Mouse: Nf2fl/fl;Wwc1fl/fl;Wwc2fl/fl (C57/BL6J background)	Qi et al.31	N/A
Mouse: Nf2fl/fl;Trp53fl/fl (C57/BL6J background)	This paper	N/A
Mouse: Nf2fl/fl;Trp53fl/fl;Wwc1fl/fl;Wwc2fl/fl (C57/BL6J background)	This paper	N/A
Mouse: SuperHippofl/fl (C57/BL6J background)	Qi et al.30	N/A
Mouse: Nf2fl/fl;Trp53fl/fl;SuperHippofl/+ (C57/BL6J background)	This paper	N/A
Oligonucleotides
WWC1 siRNA: 5′-GGUUGGAGAUUACUUCAUAGA-3′	This paper	N/A
WWC2 siRNA: 5′-GGAUCUUCAUCCAGUACUAAA-3′	This paper	N/A
WWC3 siRNA: 5′-GGAUAUUCAACAAAUACAAAG-3′	This paper	N/A
RT-qPCR primer GAPDH forward 5′-ATGGGGAAGGTGAAGGTCG-3′	This paper	N/A
RT-qPCR primer GAPDH reverse 5′- GGGGTCATTGATGGCAACAATA-3′	This paper	N/A
RT-qPCR primer CTCF forward 5′- CCAATGACAACGCCTCCTG-3′	Yu et al.76	N/A
RT-qPCR primer CTCF reverse 5′- TGGTGCAGCCAGAAAGCTC-3′	Yu et al.76	N/A
RT-qPCR primer CYR61 forward 5′- AGCCTCGCATCCTATACAACC-3′	Yu et al.76	N/A
RT-qPCR primer CYR61 reverse 5′- TTCTTTCACAAGGCGGCACTC-3′	Yu et al.76	N/A
RT-qPCR primer ANKRD1 forward 5′- CACTTCTAGCCCACCCTGTGA-3′	Yu et al.76	N/A
RT-qPCR primer ANKRD1 reverse 5′- CCACAGGTTCCGTAATGATTT-3′	Yu et al.76	N/A
Recombinant DNA
pLVX-puro vector	Takara	#632164
PsPAX2	Didier Trono	Addgene #12260
pMD.2g	Didier Trono	Addgene #12259
AAV2/6-CMV-3xFLAG-SuperHippo-P2A-NF2-WPRE	This paper	N/A
AAV2/6-CMV-3xFLAG-NF2-WPRE	This paper	N/A
AAV2/6-CMV-3xFLAG-SuperHippo-WPRE	This paper	N/A
Software and algorithms
GraphPad Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/
ImageJ	NIH	https://imagej.nih.gov/ij/
R (version 3.6.2)	Open Source	https://www.r-project.org/