Figure 2.
Strong antitumor effect of ADV combined with Tα1 in multiple tumor models
(A) Schematic representation of experimental design and treatment timeline. WT mice were injected subcutaneously with tumor cells. When the tumor volume reached approximately 50–100 mm3, the mice were randomly divided into different groups and treated with an intra-tumoral injection of 0.1 mL of PBS or ADV (2 × 108 PFU) every 2 days for a total of three times. Tα1 (0.25 mg/kg) was injected subcutaneously into the peritumoral site once daily starting with the first dose of ADV; s.c., subcutaneous; i.t., intra-tumoral.
(B–D) 4T1 tumor-bearing mice were treated with different administrations, starting on day 10 after tumor injection (n = 6–9 biological replicates). (B) Tumor growth. (C) Survival. (D) Body weight.
(E–G) CT26 tumor-bearing mice were treated with different administrations, starting on day 10 after tumor injection (n = 9–10 biological replicates). (E) Tumor growth. (F) Survival. (G) Body weight.
(H–J) H22 tumor-bearing mice were treated with different administrations, starting on day 5 after tumor injection (n = 10 biological replicates).
(H) Tumor growth.
(I) Individual tumor growth.
(J) Body weight.
(K) Experimental schematic of H22 model, the first rechallenge model, and bilateral tumor-bearing model: The cured mice administered immunotherapies were injected subcutaneously with 5 × 106 H22 cells. The cured mice with immunological memory were again rechallenged with 5 × 106 H22 cells and the opposing flank was injected subcutaneously with 5×105 4T1 cells on day 90 after the first rechallenge, naive mice were used as a control.
(L) Tumor growth of H22 model mice in the first rechallenge model (n = 3–6 biological replicates); CR, complete response.
(M) Survival of mice from Figures 2H–2J (n = 10 biological replicates).
(N) Tumor growth of the bilateral tumor-bearing model (n = 4–6 biological replicates); CR/IM, complete response with immunological memory.
The data are shown as the means ± SD. NS, no significant difference; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.